Loading...

Summer Research Fellowship Programme of India's Science Academies

DESIGN AND DEVELOPMENT OF ORAL GENE DELIVERY SYSTEM USING LYSOZYME NANOPARTICLES

SYED TAZIB RAHAMAN

B. Pharm, GITAM (Deemed to be University), Visakhapatnam, A.P, India 530045.

DR. CHITTARANJAN PATRA

Principal Scientist, Department of Applied Biology, CSIR-Indian Institute of Chemical Technology, Uppal Road, Tarnaka, Hyderabad, Telangana, India 500007. 

ABSTRACT

Oral gene delivery is a new area of gene therapy to treat diseases in the gastro intestinal track. The major challenge in oral gene delivery is acidic pH of gastric juice (1.5 to 3.5) and the presence of digestive enzymes which limits the potential of therapeutic agents. In this regard, nanotechnology offers gene delivery platforms to overcome the aforesaid barriers and achieve efficient gene transfection. Researchers have developed various metal, polymeric and lipid based nanoparticles for oral route delivery. However, protein nanoparticles based gene delivery vehicles for the treatment of gastro intestinal diseases are not well addressed. The present work demonstrates the design and development of a gene delivery system based on lysozyme protein nanoparticles that are stable in acidic pH and are able to deliver genes via the oral route.

Screen Shot 2018-06-22 at 7.24.49 PM.png
    Oral gene delivery of lysozyme protein nanoparticles

    Keywords: Nanoparticle, Protein NPs, Lysozyme, Oral gene delivery,SEM, Animal Imaging,DNA.

    More
    Written, reviewed, revised, proofed and published with