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Summer Research Fellowship Programme of India's Science Academies

Analysis of mutational profiles of SARS-CoV-2 structural and non-structural proteins with emphasis on spike protein variants

Saptarshi Bhattacharyya

KIIT SCHOOL OF BIOTECHNOLOGY, KIIT -DU. Patia, Bhubaneswar 751024

Dr. Raghunath Chatterjee

Associate Professor, INDIAN STATISTICAL INSTITUTE 203 B.T. ROAD , KOLKATA 700108

Abstract

SARS-CoV-2 has very recently posed a potential threat to humanity due to its very rapid rate of mutations and repairing mechanism. The spread of this virus is considered to occur from Wuhan, China in December 2019. It was initially labeled as 2019-nCoV and later, officially designated as SARS-CoV-2 by the International Committee on Taxonomy of Viruses (ICTV). Characterized by high rates of transmission, it has already spiraled out of control, reportedly infecting at least 88 million humans while nudging 2 million deaths world-wide. The virus is constantly evolving and attaining higher rates of stability and transmissibility through acquiring mutations in its genome. Therefore, aim of our study is to analyse the mutational profiles of SARS-CoV-2 isolates.Analysis of the mutational profiles in individual SARS-CoV-2 proteins will allow us to look into the rates of mutations associated with each protein. It is instrumental in identifying the proteins that are resistant to changes as well as the ones that have a greater proclivity towards incorporating mutations. Assessment of the proclivities of the different viral proteins towards incorporating mutations would be beneficial in regards to vaccine development.

Keywords: D614G Mutation, S1 & S2 Spike proteins, MEGA X (10.0), Multiple Sequence Alignment, Phylogenetic tree,synonymous and non- synonymous mutations.

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