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Summer Research Fellowship Programme of India's Science Academies

Expression analysis of micro RNAs and their target genes associated with root architecture in tomato (Solanum lycopersicum)

M.Swaathy

Agricultural College & Research Institute, TNAU, Coimbatore- 641003

Prof. Dr. M V Rajam

Department of Genetics, University of Delhi-South Campus, New Delhi-110021

Abstract

The miRNAs are short non-coding RNAs found in many organisms, including plants that regulate gene expression either by cleavage or translational blockage of the target mRNA. The roots of the plants differentially express certain miRNAs in response to internal/external cues to fine regulate the expression levels of the target gene involved in root architecture. These are also involved in maintaining root homeostasis, biotic and abiotic interactions of root with the external elements. Adverse environmental conditions can up-regulate or down-regulate the expression of these miRNAs to bring about fine tuning of the miRNA target genes which are involved in stress tolerance. In view of the importance of miRNAs in regulating the root architecture in plants, the present study has been undertaken with the aim to investigate the expression analysis of miRNAs-miR166c, miR172a, miR847 and miR5303, their target genes involved in root architecture in tomato. It is found by the study that miRNAs-166C, 172a are present comparatively in higher amounts in roots. This can be concluded that these miRNAs may be involved in functions governing root architecture. But, transgenic expression analysis has to be done to verify.

Keywords: stem loop, RT-PCR, qPCR

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