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Summer Research Fellowship Programme of India's Science Academies

Production and Characterization of Mcl-PHA using Pseudomonas sp

Harender Yadav

Department of Plant Biology and Biotechnology, Madras Christian College, East Tambaram, Tambaram, Chennai, Tamil Nadu 600059

Dr. Saugata Hazra

Assistant Professor, Department of Biotechnology, IIT Roorkee, Roorkee - Haridwar Highway, Roorkee, Uttarakhand 247667

Abstract

Traditional plastic and its degradation, has been a nuisance to environment and to humans for millennia together. To counterattack this problem, the research is going on production and characterisation of Polyhydroxyalkanoates (PHA) which can be used as bioplastics. PHA are biodegradable biopolymers which are produced by gram positive and gram-negative bacteria during stress conditions like abundance of carbon source or limited nitrogen, oxygen or phosphorous content. It is produced in intercellular granules called carbonosomes. Pseudomonas strains were screened using Nile Red and Sudan Black B dye for PHA accumulation which showed Yellow fluorescence and blackish blue staining respectively. PHA accumulation in the cells was confirmed. Later, MSM medium was optimized for better biomass production. Growth parameters like pH (6-9), Temperature (25-40 °C) and glucose concentration (1-4%) were optimized with respect to higher biomass production. It was found that at pH 7 and 4% glucose concentration incubated at 37 °C, Pseudomonas strains shows highest biomass production in 24 hr. Optimized media can be further used for mass production of PHA at in vitro level.

Keywords: Pseudomonas sp., PHA, green plastic, biodegradable plastic, bioplastic

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