Agrobacterium tumefaciens - mediated Fungal Transformation in Chilli Plants
Colletotrichum truncatum, also known as C. capsici, belongs to the family Glomerellaceae (Glomerellales, Sardiomycetes) under the phylum Ascomycota. It causes fruit rot or anthracnose on many economically important plants including cereals, legumes, chillies, soybean, etc. (Than et al. 2008). Chilli anthracnose is characterized by dark, sunken necrotic lesions with concentric rings of acervuli-containing conidia. Very scant information is available about its pathogenicity-related genes and its mode of action. In the present study, we plan to generate a library of C. truncatum transformants using a random insertional mutagenesis strategy through Agrobacterium tumifaciens-mediated transformation (ATMT) for identification and characterization of the putative pathogenicity gene. We observed hygromycin-resistant colonies which are being tested for their pathogenic effects on chilli plants. Identification of pathogenicity genes and the molecular mechanisms of plant-fungal interactions would help in controlling C. truncatum-related plant infections in a more efficient and promising way.
Keywords: C. truncatum, ATMT, random insertional mutagenesis, pathogenicity genes, pathogenicity assay.