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Summer Research Fellowship Programme of India's Science Academies

Evaluation of antiviral activity of marketed Formulations - Carica papaya L. Leaf Extract

K Muddukrishnaiah

Sanjo College of Pharmaceutical Studies, Palakkad, Kerala.

Dr. Sujatha Sunil, PhD

International Center for Genetic Engineering and Biotechnology (ICGEB), Aruna Asif Ali Marg, New Delhi.

Abstract

Chikungunya (CHIK), a viral fever spread by Aedes mosquito results in extreme morbidity rates in affected populations and is a major public health concern in India. At present, recent vaccines or formulations prescribed by physicians can only provide relief for the pyretic and post pyretic phase of the disease. Antiviral activity of Carica Papaya (CP) Leaf Extract and Carica Papaya Leaf supplemented with polyherbal extract (CP mix) marketed formulations was studied. CP and CP mix formulations each 100 mg/ml were separately dissolved in distilled water and filtered by using 0.22 micron filters.and the filtrate was stored at 4°C. Cytotoxicity of the CP and CP mix formulation were evaluated on the Vero cell lines using MTT assay. Antiviral activity of CP and CP mix against CHIKV was determined by using Pre-incubation, Post treatment and Protective effect assays. Aqueous extracts of CP and CP mix showed non-cytotoxic effects (0.35mg/ml) to Vero cells. Antiviral activity of CP and CP mix concentration range 0.35mg/ml to 0.0341mg/ml. This result indicates that the CP and CP mix formulated aqueous extract do not possess inhibitory action against CHIKV growth under in-vitro conditions. This is a preliminary study report on antiviral activity of Carica Papaya, an Indian medicinal plant against CHIKV. Further studies are required to know the mechanism of action of the exhibited antiviral activity.

Keywords: Carica Papaya L.; Anti-CHIKV activity; Vero cell line

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