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Summer Research Fellowship Programme of India's Science Academies

In-vivo tracking of Mesenchymal stem cells in animal model (mice)

Riya Suri

University of Jammu, Vikram Chownk, Jammu Tawi, 180006

Sujata Mohanty

DBT-Centre of Excellence for Stem Cell Research, All India Institute of Medical Sciences, New Delhi, 110029

ABSTRACT

Mesenchymal stem cells (MSCs) contribute to the recovery of tissue injury. The distribution of bone marrow derived MSCs injected in mice, stained with different dyes i.e. PKH 26, Xenolight Dir was investigated. Different routes of injection i.e. IP, IV and SC were also investigated to see which gave better results. A high sensitivity imaging system i.e. IVIS imaging system (Lumina XR), caliper, USA was used to detect fluorescence. The instrument was absolutely calibrated so that animal images were analyzed in physical units of radiance. IN-VITRO, IN-VIVO, EX-VIVO studies were done to detect the fluorescence. IHC (Immunohistochemistry) and FISH (Fluorescence In Situ Hybridization) were used to confirm the presence of cells.

Keywords: Immunohistochemistry, Fluorescence In Situ Hybridization

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