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Summer Research Fellowship Programme of India's Science Academies

Molecular Analysis of Transgenic CRISPR-edited Rice Lines for Manipulation of Salt-inducible Gene

Sanjay Kumar Pradhan

Department of Agricultural Entomology, University of Agricultural Sciences, Gandhi Krishi Vignan Kendra, Bangalore-560065

Dr. Surekha Katiyar-Agarwal

Department of Plant Molecular Biology, University of Delhi, New Delhi-110021

Abstract

Plants exhibit a variety of abiotic stress-responsive adaptations that enable them to tolerate adverse climatic conditions; these involve physiological, biochemical and molecular processes. Salt stress is a major threat to agriculture across the world. The salt stress responses are mediated by different stress-responsive and developmental genes which alter the metabolic pathways as well as the physiology of plants. Stress-activated transcription factors regulate gene expression, which finally results in changes at the protein level. Different signaling pathways like Salt Overly Sensitive (SOS), Mitogen-Activated Protein Kinase (MAPK) and Reactive Oxygen Species (ROS) signaling pathways play a pivotal role in this process. In this study, a salt-inducible gene was found and later characterized. Rice genome editing by CRISPR-Cas9 mechanism was done by using pRGEB31 plasmid to obtain transgenic rice lines; efforts were made to isolate total genomic DNA and perform molecular analysis of these transgenic rice lines.

Keywords or phrases: CRISPR-Cas9, Transgenic lines, Gradient PCR, Transformation, Rice

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