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Summer Research Fellowship Programme of India's Science Academies

Genetic Diversity Analysis of Different Genotypes of Vigna mungo using CDDP and DAMD Markers

Lukapriya Dutta

Department of Botany, Gauhati University, Guwahati, Assam, 781014

Prof. Amita Pal

Emeritus Scientist, Division of Plant Biology, Bose Institute, Kolkata,700054

ABSTRACT

Blackgram (Vigna mungo) is one of the widely cultivated crops in Asian countries. It is an important source of protein, carbohydrates, minerals and dietary fibres. Out of all other species of the family, less research work has been carried out on blackgram. A molecular marker is a fragment of DNA that is associated with a certain location within the genome.  It is a useful tool for the study of genetic diversity. Different molecular markers such as RAPD, AFLP etc. were previously used in several biodiversity studies. But these techniques have certain disadvantages due to which new PCR based marker systems have been developing in recent years. CDDP and DAMD are two widely used PCR based marker systems. In the first case a single primer is used both forward as well as reverse. The CDDP primers tagged with the conserved regions in the genome. In case of DAMD primers, they amplify hyper variable DNA sequences, minisatellites of DNA. In this study, 4 CDDP and 3 DAMD primers were used to study the genetic diversity in some selected accessions of V. mungo. The genomic DNA was isolated by using CTAB method. Mastermix was prepared for PCR amplification and PCR cycles were programmed according to the annealing temperature of different primers. The amplicons were visualized in 2% agarose gel stained with ethidium bromide. Total number of amplified bands and total number of polymorphic bands were calculated in each case and percentage polymorphism was calculated. The average polymorphism was 54.2% for CDDP and ~52% for DAMD.

Keywords: Molecular markers, Polymorphism, Conserved DNA, Minisatellite DNA, PCR

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