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Summer Research Fellowship Programme of India's Science Academies

Investigation on the comparative therapeutic efficiency of bioactive polyphenols from Annona reticulata and immunomodulatory proteins of Hymenolepis diminuta on colitis.

Vinod Kumar D

Department of Biotechnology, P.E.S University, Bangalore 560085

Dr. S. P. Sinha Babu

Professor, Department of Zoology, Visva Bharati University, Santiniketan 731235

Abstract

The aim of the present study is to elucidate the therapeutic potential of immunomodulatory proteins of Hymenolepis diminuta and polyphenols of Annona reticulata and the analysis of their comparative efficiencies on anti-colitis activity. Colitis is a major problem around the world and it plays a significant role in inflammatory bowel disease whose exact etiology and pathology remain unknown. There is increasing evidence suggesting that parasite infection has the ability to ameliorate other diseases involving inflammation and mucosal immune dysregulation. Polyphenols have also been proven to have major anti-oxidant, anti-tumor, anti-inflammatory properties, being bio-active secondary metabolites rich in fruits and vegetables. It is mandatory to elucidate their pharmacological activities in treating colitis. The major objectives of this research are to optimize the extraction parameters for polyphenols from Annona reticulata; isolation, partial purification and characterization of proteins from Hymenolepis diminuta, and lastly, to study the effects of polyphenols on acetic acid-induced colitis on a rat model. Colitis was assessed by the Disease Activity Index (DAI), and microscopic and histological damage scores. In our study, we have found that there is a significant reduction in disease activity index and histological damage scores after treating the colitis induced rats with compounds of interest such as protein extracts, polyphenols, and Dexamethasone. The architecture of epithelial tissue in rat colon showed major changes when compared with controls. Hymenolepis diminuta proteins are found to have greater therapeutic efficiency than polyphenols from Annona reticulata and commercially available steroids. Considering the many disadvantages of current treatment for colitis, this approach of drug development can have a major impact on treating colitis and it is also hoped that further optimization and standardization of this method can lead to increase in efficiency and to the full realisation of the potential of these compounds in alleviating colitis.

Keywords: Inflammatory bowel disease, Anti-colitis activity, natural therapeutics

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