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Summer Research Fellowship Programme of India's Science Academies

Isolation, Differentiation and Characterization of Osteoclasts from Mouse Bone-Marrow

T K Padmavathy

Department of Biotechnology, Pondicherry University, Puducherry 605014

Dr. Mohan R Wani

Scientist-G, National Centre for Cell Science, University of Pune Campus, Pune 411007

Abstract

The bone is a rigid connective tissue made up of 90% collagen protein. The main function of the bone is to provide structure and support to all the vital organs of the body and it helps in movement. Osteoblast, osteoclast and osteocytes are the three major types of cells constructing this bone tissue. They are mainly involved in maintaining bone homeostasis by removing the old bone cells and forming new ones followed by a mineralization processes. Osteoclast cells are large in size upto 200µm in diameter and they are multinuclear in nature. During the initial days of osteoclast research, mature osteoclast cells are isolated from chicken long bones. With the discovery of regulatory roles of macrophage colony - stimulating factor and RANKL in osteoclast formation, researchers used recombinant human and mouse M-CSF and RANKL for the culturing of osteoclast from monocyte –macrophage precursor cell in vitro method. Since these three cells are mainly involved in regulating bone homeostasis, study of their isolation, differentiation and characterization are essential to understand the more complicated bone metabolic pathways involved in bone degenerative diseases like osteoarthritis, rheumatoid arthritis, osteoporosis etc.,

Keywords: Bone marrow, osteoclast, Monocyte -macrophage precursor cell, M-CSF, RANKL

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