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Summer Research Fellowship Programme of India's Science Academies

The Antioxidant Activity of Methanolic extract of Plant A in HABP1 transfected fibroblast cells

N Pooja

Karpagam University, Pollachi Main Road, Eachanari Post, Coimbatore 641021 (Tamil Nadu)

Prof Kasturi Datta

Jawaharlal Nehru University, New Mehrauli Road, Delhi 110067

Abstract

The hyaluronic acid binding protein (HABP1) has been shown to regulate a variety of functions like apoptosis, immunological reactions, splicing mechanism, cell differentiation and cancer progression. It is identical to splicing factor associated protein p32 and gC1qR a receptor protein in globular head of complement component. The earlier experiments conducted in HABP1 transfected F111 fibroblast cell line showed increased reactive oxygen species. Since reactive oxygen species are known to cause several detrimental effects to cells including DNA damage, early apoptosis, tumorigenesis, cancer progression and also show adverse effects in various infectious diseases, the scavenging of these reactive oxygen species may prove beneficial to control many of its damaging effects. Several commercially available antioxidants are available in the market but the increasing awareness of naturally occurring antioxidants demands the revival of our natural medicinal resources. Medicinal plants have been used from the early periods to cure diseases without causing any side effects. The demand for using natural medicines is increasing due to the side effects caused by the synthetic medicines. So here we have attempted to use the well known medicinal plant Plant A to eliminate the reactive oxygen species and also the effects of HABP1 in senescene. We conducted flourimetry assays and protein estimation assays to determine the reactive oxygen species. We also conducted immunofluorescence assay for the p16, a marker for senescence and β-gal assay, another marker for senescence. So, the methanolic extract of Plant A, a well known medicinal plant used in various ayurvedic medicines was used to control the level of reactive oxygen species in the cells.

Keywords: Reactive oxygen species, HABP1, senescence, apoptosis, antioxidants, cancer progression

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