The Fascinating World of Lab Immunology
The study of the molecular and cellular components that comprise the immune system, including their function and interaction, is the central science of immunology. Undoubtedly, in recent years the advancements in immunology have critically facilitateddevelopment in biomedical research. The past 8 weeks have given me a great insight into the fascinating world of lab immunology. As I am new to the field of immunology, I spent my time learning the basic techniques used in a Clinical Immunology lab. In the last 2 weeks I tried designing a small project and carried out a few experiments for the same. The project was about checking the effect of Synovial Fluid of Rheumatoid Arthrirtis on B cell subsets of healthy individuals. This experience has greatly helped my appreciate the time and efforts that a researcher takes to improve every aspect of the project he is working on.
Keywords: Clinical Research Laboratory techniques, Rheumatoid arthritis, B cell subsets
The synovial fluid (SF) contains a variety of cytokines, chemokines, growth factors and lipid-derived mediators, which potentially mediate B cells effector functions. Therapeutic depletion of B cells results in important improvement in controlling disease activity in rheumatoid patients. Therefore we investigated the effect of synovial fluid of RA patients on Bcell subsets. For this study, Synovial fluid of 3 RA patients was collected.Heparinated blood from a healthy individual was collected and treated with 10% SF in RPMI medium for 24 h.B cells subsets were identified by flow cytometry.