INTO THE ABODE OF IMMUNOLOGICAL TECHNIQUES
Keywords: Immunology, Techniques, Rheumatoid Arthritis, Th17, Treg, Synovial fluid.
The synovial fluid of Rheumatoid Arthritis patients contains many proinflammatory cytokines like IL-1β, IL-23, IL-15, IFN-ϒ, TNF-α etc. The peripheral blood sample of RA patients was found to have disturbed Th17/Treg balance, due to the expansion of Th17 cells and Th17-related proinflammatory cytokines. Decreased frequency of Treg cells have also been observed in RA. Th17 causes an inflammatory response by release of proinflammatory cytokines, whereas Tregs have the role of immunotolerance, suppressing inflammatory activities. Th17 /Treg balance is very important for a healthy immunoregulation. A disruption in this pattern can lead to the development of autoimmunity. This work aims to investigate the effect of RA synovial fluid on the Th17/Treg ratio in healthy blood sample. This study infers to know whether the proinflammatory cytokines present in RA synovial fluid cause the polarization of T cells and leads to the expansion of Th17 cells in a healthy individual by assessing its markers in Flow Cytometer.