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Summer Research Fellowship Programme of India's Science Academies

Effect of hyperthermia on endothelial cells

Ms. Swetha S.

Indian Institute of Technology - Jodhpur (IIT-J), NH 65, Nagaur Road, Karwar, Jodhpur District, 342037

Dr. Kumaravelu Jagavelu

Senior Scientist, Pharmacology Division, Central Drug Research Institute, CSIR-CDRI, Jankipuram Extension, Lucknow, 226031

Abstract

Inflammation is the protective immune response elicited by injury or infection to destroy or dilute both the infectious agent and the injured tissue. Endothelial cells, the foremost gate keepers for the peripheral tissue from the vascular circulation, play a critical role in initiation, amplification and resolution of inflammatory response. The pro and anti-inflammatory signaling pathways in endothelial cells are tightly regulated and are well characterized. On the other hand, hyperthermia being the surrogate marker for inflammation might lead to endothelial cell damage and is poorly understood. Hence, we sought to study the thermal sensitivity of endothelial cells invitro and observe the mechanism by which they respond to high temperature by analyzing the expression of inflammatory markers like vWF, ICAM-1 and E-Selectin. Our analysis has concluded that inflammatory genes are up regulated only upon an endotoxin induction, while hyperthermia can augment the endotoxin-induced inflammation, and hyperthermia alone cannot trigger the expression of inflammatory genes.

Keywords: Endothelial cells, hyperthermia treatment, inflammation and gene expression analysis

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