Dorka Vijaya Kumari Beri
Lecturer in Biochemistry, Govt. Degree College for Women (A), Guntur. 522 001
Prof. Hari Sharan Misra
Head, Molecular Biology Division, BARC, Mumbai. 400 094
Dr. Yogendra Singh Rajpurohit
SO/F, Molecular Biology Division, BARC, Mumbai 400 094
Deinococcus radiodurans is known to be one of the toughest organisms on earth exhibiting extreme radioresistance and has the capacity to reconstruct its genome by its well-organized DNA repair mechanisms. One of the important proteins for reassembling genomes having double stranded breaks is PprA (Pleiotropic protein promoting DNA repair). In vitro molecular studies on DNA metabolic proteins require a very high-quality preparation of recombinant protein. A pleiotropic protein (PprA) involved in radiation resistance is found to be a member of a multiprotein complex involved in DNA repair in radioresistant bacterium D. radiodurans. A protocol was optimized for the purification of recombinant PprA protein by polymin-P method.
Keywords: PprA, Protein purification, Radioresistance