Loading...

Summer Research Fellowship Programme of India's Science Academies

Overexpression & Purification of PprA protein involved in DNA repair from Deinococcus radiodurans

Dorka Vijaya Kumari Beri

Lecturer in Biochemistry, Govt. Degree College for Women (A), Guntur. 522 001

Prof. Hari Sharan Misra

Head, Molecular Biology Division, BARC, Mumbai. 400 094

Dr. Yogendra Singh Rajpurohit

SO/F, Molecular Biology Division, BARC, Mumbai 400 094

Abstract

Deinococcus radiodurans is known to be one of the toughest organisms on earth exhibiting extreme radioresistance and has the capacity to reconstruct its genome by its well-organized DNA repair mechanisms. One of the important proteins for reassembling genomes having double stranded breaks is PprA (Pleiotropic protein promoting DNA repair). In vitro molecular studies on DNA metabolic proteins require a very high-quality preparation of recombinant protein. A pleiotropic protein (PprA) involved in radiation resistance is found to be a member of a multiprotein complex involved in DNA repair in radioresistant bacterium D. radiodurans. A protocol was optimized for the purification of recombinant PprA protein by polymin-P method.

Keywords: PprA, Protein purification, Radioresistance

More
Written, reviewed, revised, proofed and published with