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Summer Research Fellowship Programme of India's Science Academies

Screening of transposon mutagenized library of E.coli to identify target genes for Mycobacteriophage proteins.

Manisha Priya

M.Sc. Applied Microbiology, Department of Botany, Institute of Science, Banaras Hindu University, Varanasi

Dr. Ranjan Sen

Staff Scientist, Transcription lab, Centre for DNA Fingerprinting and Diagnostics, Hyderabad, India

Abstract

Mycobacterial diseases are the major cause of mortality around the world. According to WHO in 2017, 10 million people get infected with tuberculosis. Resurgence of tuberculosis and the rapid emergence of drug resistant strain of Mycobacteria have made it necessary for scientists to look for alternative approach other than antibiotics and vaccines.

Many scientists are trying to develop phage based tools for the treatment of Mycobacterial diseases. Phage therapy is the use of bacteriophage to treat bacterial infections and it was first used by Felix d Herelle. Currently, phage therapy is used in Eastern Europe and Countries of the former Soviet Union for the treatment of various bacterial infections.

Mycobacteriophage are viruses that infect a Mycobacterial host. Till date, 10,343 Mycobacteriophages have been isolated using M. smegmatis mc2155 as host, out of which 1716 have been completely sequenced. Mycobacteriophage are natural killers of Mycobacteria so the use of mycobacteriophage proteins has been highly assesed for the treatment of Mycobacterial infections.

In this project an attempt has been made to find the target gene for a mycobacteriophage protein which has already shown lethality in M. smegmatis, M. bovis and E. coli. Transposon mutagenesis approach is used to find the target gene in E. coli. E. Coli is choosen for the study because it it easy for geneic manipulation.

Keywords: Transposon mutagenesis, Screening of transposon mutagenized library

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