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Summer Research Fellowship Programme of India's Science Academies

Study of protein-protein interactions between PfAlba1 and PfGCN5 in Plasmodium falciparum

Hari Priya Saripalli

Department of Biochemistry, Andhra University, Visakhapatnam 530003

Dr. Krishanpal Karmodiya

Assistant Professor, Department of Biology, Indian Institute of Science Education and Research (IISER) Pune 411008

Abstract

Acetylation Lowers Binding Affinity protein 1 (PfAlba1) is a DNA-RNA binding protein known to bind to RNA during the trophozoite stage of the parasite Plasmodium falciparum's life cycle and is involved in post-transcriptional regulation, which plays an important role in expression of invasion genes in Plasmodium falciparum. Acetylation of this protein lowers its binding affinity to RNA which is then released. Preliminary data from our lab suggests that GCN5 acetyl transferase, involved in stress response, interacts with PfAlba1. It was confirmed that PfAlba1 interacts with PfGCN5. It has been found that the protein does not interact with recombinant GCN5 alone having HAT and bromodomains. Further, the protein interacts with PfGCN5 in the parasite lysate suggesting the possible binding of PfAlba1 to regions other than the HAT and bromodomain or the involvement of other proteins or factors to faciltate the interaction. This report contributes to the understanding of the connection between parasite stress response and post-transcriptional regulation by establishing the interaction between PfAlba1 and PfGCN5. This may give insights into the mechanism of epigenetic regulation of protein expression in response to stress.

Keywords: Plasmodium falciparum, epigenetics, histone acetyl transferase, PfGCN5, PfAlba1, Interaction assays.

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